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A Laboratory Guide to Genomic Sequencing: The Direct Sequencing of Native Unclon
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- Buy It NowA Laboratory Guide to Genomic Sequencing: The Direct Sequencing of Native Unclon
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eBay item number:364693755468
Item specifics
- Condition
- ISBN-13
- 9783764319250
- Book Title
- A Laboratory Guide to Genomic Sequencing
- ISBN
- 9783764319250
- Publication Year
- 1987
- Series
- Biomethods Ser.
- Type
- Textbook
- Format
- Trade Paperback
- Language
- English
- Publication Name
- Laboratory Guide to Genomic Sequencing
- Item Length
- 10in
- Publisher
- Springer Basel A&G
- Item Width
- 7in
- Item Weight
- 16.8 Oz
- Number of Pages
- 164 Pages
About this product
Product Information
A Safety Considerations Genomic sequencing involves a number of hazardous steps, such as high current, high voltage, radioactive and highly toxic chemicals. It is, therefore, absolutelyessen- tial that the instructions of equipment manufacturers be followed and that particular attention is paid to the local and federal safety regulations. INTRODUCTION 9 B Introduction During the cloning of genomic DNA many of its characteristics are perma- nently lost. It was therefore necessary to develop a new technique that would give us a closer look at a gene in its normal environment. The powerful technique of genomic sequencing, first described by Church and Gilbert (1984) now makes it possible to have a precise view of a given DNA sequence in a chromosome. This method combines the chemical DNA-sequencing procedure of Maxam and Gilbert (1980) with the detection of DNA sequences by electroblotting and indirect end-labeling by hybridization. Besides studies on the methylation state of single bases in a given gene (Nick et al., 1986; Saluz and Jost, 1986; Saluz et al., 1986), genomic sequencing can also be used to study specific DNA-protein interactions in vivo (Church et al., 1985; Giniger et al., 1985; Becker et al., 1986; Ephrussi et al., 1985; Martin et al., 1986; Nick et al., 1986; Zinn and Maniatis, 1986).
Product Identifiers
Publisher
Springer Basel A&G
ISBN-10
3764319259
ISBN-13
9783764319250
eBay Product ID (ePID)
4474312
Product Key Features
Publication Name
Laboratory Guide to Genomic Sequencing
Format
Trade Paperback
Language
English
Publication Year
1987
Series
Biomethods Ser.
Type
Textbook
Number of Pages
164 Pages
Dimensions
Item Length
10in
Item Width
7in
Item Weight
16.8 Oz
Additional Product Features
Number of Volumes
1 Vol.
Lc Classification Number
H1-99
Table of Content
I. Introduction.- A Safety Considerations.- B Introduction.- C The Principle of Genomic Sequencing.- II Theoretical Background.- A Basic Theoiy of Genomic Sequencing.- B Flow Diagram.- III Experimental.- 1 Isolation of Genomic DNA.- 2 Restriction Digest of Genomic DNA.- 3 Chemical Sequencing Reactions on Restricted DNA.- 4 Separation of Reaction Products on a Sequencing Gel.- 5 Electrotransfer to Nylon Membranes.- 6 Immobilization of DNA on a Nylon Membrane.- 7 Prehybridization and Hybridization of Immobilized DNA with Labeled Single-Stranded DNA Probes.- 8 Processing of the Hybridized Filters.- 9 Autoradiography and Photography.- 10 Cloning of DNA Probe in M13.- 11 Large-Scale Preparation of Cloned DNA in M13.- 12 Synthesis of Oligonucleotide Primers and Single-Stranded Labeled Probes.- 13 Purification of Labeled Single-Stranded Probes.- IV Trouble-Shooting Guide and Examples.- V Appendix.- VI Bibliography.
Copyright Date
1987
Topic
Life Sciences / Cell Biology, Life Sciences / Genetics & Genomics
Intended Audience
Scholarly & Professional
Illustrated
Yes
Genre
Non-Classifiable, Science
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eBay item number:364693755468
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Item as described, good price, well packaged, arrived slightly later than hoped (not in time for Christmas ) but the order was placed in a very busy shipping period. No issue with the seller’s speed of response and sending the item. Great seller!
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I was pleasantly surprised by how soon I received this textbook given that it shipped from outside the U.S. It was packaged well with no damage. The price was right. And I greatly appreciated the communication informing me where the book was shipping from and including a tracking number. Overall, a positive experience, - online shopping the way it should be. I've bought books from this seller several times now without any complaints and will surely buy from them again. Thanks a lot!
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Quality and hard to find book that won't be released 'til January 2025 in the U.S.A.
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